Effects of simulated anaemia on the blood chemistry of Atlantic cod, Atlantic halibut, and Atlantic salmon
Abstract
Abstract
Anaemia is a common pathology associated with many diseases and infections, though its
physiological effects are poorly understood. This project aimed to develop a methodology
for inducing a stable experimental anaemia in Atlantic cod, halibut, and salmon, and thereby
investigate the physiological impact of anaemia.
Atlantic cod, halibut, and salmon were rendered progressively anaemic by means of
intraperitoneal injections of 3 μg·g-1 phenylhydrazine in a dimethyl sulfoxide vehicle,
dissolved in Cortland’s saline. One injection was sufficient to induce anaemia in Atlantic
cod, while two injections, one week apart, provided an acceptable reduction in haematocrit
and haemoglobin for both Atlantic halibut and salmon.
There was a significant reduction in plasma lactate in the phenylhydrazine-injected group of
Atlantic cod, and plasma glucose was significantly reduced in anaemic and control fish for
both Atlantic halibut and salmon. Additionally, there was a significantly lower concentration
of plasma sodium and chloride in the Cortland’s saline-only control group of Atlantic halibut.
Peripheral blood smear analysis showed no significant differences in leukocyte proportions in
any of the species investigated, but there was a marked increase in immature erythrocytes in
the anaemic groups of all species.
Description
Mastergradsoppgave i havbruk - Høgskolen i Bodø, 2009