dc.contributor.author | Ahmad, Sharmila | |
dc.contributor.author | Lyngman, Lise Kirstine | |
dc.contributor.author | Mansouryar, Morteza | |
dc.contributor.author | Dhakal, Rajan | |
dc.contributor.author | Agerholm, Jørgen Steen | |
dc.contributor.author | Khanal, Prabhat | |
dc.contributor.author | Nielsen, Mette Olaf | |
dc.date.accessioned | 2021-01-27T14:35:50Z | |
dc.date.available | 2021-01-27T14:35:50Z | |
dc.date.created | 2020-10-26T09:42:02Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Ahmad, S., Lyngman, L. K., Mansouryar, M., Dhakal, R., Agerholm, J. S., Khanal, P. & Nielsen, M. O. (2020). Depot and sex-specific implications for adipose tissue expandability and functional traits in adulthood of late prenatal and early postnatal malnutrition in a precocial sheep model. Physiological Reports, 8(19). doi: | en_US |
dc.identifier.issn | 2051-817X | |
dc.identifier.uri | https://hdl.handle.net/11250/2725036 | |
dc.description.abstract | The aim was to investigate long‐term, tissue and sex‐specific impacts of pre and postnatal malnutrition on expandability and functional traits of different adipose tissues. Twin‐pregnant ewes were fed NORM (~requirements), LOW (50% of NORM) or HIGH (150%/110% of energy/protein) diets the last 6 weeks prepartum (term ~147‐days). Lambs received moderate, low‐fat (CONV) or high‐carbohydrate‐high‐fat (HCHF) diets from 3 days until 6 months of age, and thereafter CONV diet. At 2½ years of age (adulthood), histomorphometric and gene expression patterns were characterized in subcutaneous (SUB), perirenal (PER), mesenteric (MES), and epicardial (EPI) adipose tissues. SUB had sex‐specific (♂<♀) upper‐limits for adipocyte size and cell‐number indices, irrespective of early life nutrition. PER mass and contents of adipocytes were highest in females and HIGH♂, whereas adipocyte cross‐sectional area was lowest in LOW♂. Pre/postnatal nutrition affected gene expression sex‐specifically in SUB + PER, but unrelated to morphological changes. In PER, LOW/LOW♂ were specific targets of gene expression changes. EPI was affected by postnatal nutrition, and HCHF sheep had enlarged adipocytes and upregulated expressions for adipogenic and lipogenic genes. Conclusion: upper‐limits for SUB expandability were markedly lower in males. Major targets for prenatal malnutrition were PER and males. LOW♂ had the lowest PER expandability, whereas HIGH♂ had an adaptive advantage due to increased hypertrophic ability equivalent to females. Fixed expandability in SUB meant PER became a determining factor for MES and ectopic fat deposition, rendering LOW♂ particularly predisposed for obesity‐associated metabolic risks. EPI, in contrast to other tissues, was targeted particularly by early postnatal obesity, resulting in adipocyte hypertrophy in adulthood. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Wiley | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Depot and sex-specific implications for adipose tissue expandability and functional traits in adulthood of late prenatal and early postnatal malnutrition in a precocial sheep model | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | © 2020 The Author(s) | en_US |
dc.subject.nsi | VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 | en_US |
dc.subject.nsi | VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480 | en_US |
dc.source.pagenumber | 18 | en_US |
dc.source.volume | 8 | en_US |
dc.source.journal | Physiological Reports | en_US |
dc.source.issue | 19 | en_US |
dc.identifier.doi | 10.14814/phy2.14600 | |
dc.identifier.cristin | 1842156 | |
dc.relation.project | Danish Council for Strategic Research: 09067124 | en_US |
dc.description.localcode | Unit Licence Agreement | en_US |