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Giulia Micallef, Phillip Cash, Jorge M. O. Fernandes, Binoy Rajan, John W. Tinsley, Ralph Bickerdike, Samuel A. M. Martin, Alan S. Bowman
Dietary Yeast Cell Wall Extract Alters the Proteome of the Skin Mucous Barrier in Atlantic Salmon (Salmo salar): Increased Abundance and Expression of a Calreticulin-Like Protein
www.plosone.org
www.plosone.org
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1 J
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576 196.0021 l
576 195.5021 l
200.0001 195.5021 l
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341.5623 182.5021 l
341.0623 183.0022 l
341.0623 195.5021 l
f*
341.5623 182.5021 m
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341.0623 183.0022 l
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458.5311 195.5021 l
f*
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341.5623 183.0022 l
458.5311 183.0022 l
f*
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576 183.0022 l
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341.5623 182.5021 m
341.5623 170.0022 l
341.0623 170.5022 l
341.0623 182.5021 l
f*
341.5623 170.0022 m
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341.0623 170.5022 l
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f*
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341.5623 170.5022 l
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f*
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576 170.5022 l
f*
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341.5623 157.5023 l
341.0623 158.0023 l
341.0623 170.0022 l
f*
341.5623 157.5023 m
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341.0623 158.0023 l
f*
459.0312 170.0022 m
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458.5311 158.0023 l
458.5311 170.0022 l
f*
459.0312 157.5023 m
341.5623 157.5023 l
341.5623 158.0023 l
458.5311 158.0023 l
f*
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459.0312 157.5023 l
459.0312 158.0023 l
576 158.0023 l
f*
341.5623 157.5023 m
341.5623 145.0023 l
341.0623 145.5024 l
341.0623 157.5023 l
f*
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(following)Tj
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[()11()]TJ
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(.)Tj
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([)Tj
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(33)Tj
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1.8482 0 Td
(adapted)Tj
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1.3833 0 Td
(CBB-stained)Tj
5.2554 0 Td
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3.0727 0 Td
(the)Tj
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(sequencing)Tj
4.7168 0 Td
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2.4378 0 Td
(trypsin)Tj
3.0387 0 Td
(\(Promega,)Tj
4.3653 0 Td
(Southampton,)Tj
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(UK\))Tj
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(37C)Tj
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1.389 0 Td
(8)Tj
0.6917 0 Td
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4.3539 0 Td
(NY,)Tj
1.8142 0 Td
(USA\))Tj
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1.7291 0 Td
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(liquid)Tj
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5.4878 0 Td
(\(LC-MS/MS\))Tj
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(analysis)Tj
3.3222 0 Td
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1.2756 0 Td
(electrospray)Tj
5.057 0 Td
(ionisation)Tj
4.2236 0 Td
(\(EIS\)-ion)Tj
3.9571 0 Td
(trap)Tj
1.8425 0 Td
(instrument.)Tj
-31.6854 -1.3039 Td
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(chromatography)Tj
6.8768 0 Td
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1.2756 0 Td
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1.2019 0 Td
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3.8211 0 Td
(3000)Tj
2.1203 0 Td
(LC)Tj
1.423 0 Td
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3.05 0 Td
(\(Dionex)Tj
3.5093 0 Td
(Ltd.,)Tj
-33.4996 -1.2982 Td
(Camberley,)Tj
4.7848 0 Td
(Surrey,)Tj
3.0727 0 Td
(UK\))Tj
1.9673 0 Td
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2.3924 0 Td
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4.7054 0 Td
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1.9899 0 Td
[()]TJ
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1.542 0 Td
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(5)Tj
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-33.029 -1.2983 Td
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1.0374 0 Td
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4.5297 0 Td
(0.04%)Tj
2.6305 0 Td
(formic)Tj
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(acid)Tj
1.8709 0 Td
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(a)Tj
0.6576 0 Td
(gra-)Tj
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(12)Tj
1.1678 0 Td
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1.4003 0 Td
[()]TJ
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1.2642 0 Td
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 442.4314 498.5007 cm
BT
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1 TL
-0.0053 Tc
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(-1)Tj
ET
Q
q
1 j
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BT
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1 TL
-0.004 Tc
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3.2372 0 Td
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4.1443 0 Td
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3.3335 0 Td
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4.0364 0 Td
(Ltd.,)Tj
2.0353 0 Td
(Coventry,)Tj
4.1669 0 Td
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4.0932 0 Td
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(1.0-min)Tj
3.3845 0 Td
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(de-convoluted)Tj
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-30.5231 -1.3039 Td
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(the)Tj
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1.9162 0 Td
(analysis)Tj
3.3222 0 Td
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Q
q
1 j
1 J
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11.9999 0 0 11.9999 200.0125 390.0472 cm
BT
/F0 1 Tf
1 TL
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3.3496 0 Td
(identification)Tj
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Q
q
1 j
1 J
0 w
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BT
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2.5398 0 Td
(database)Tj
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2.3924 0 Td
(the)Tj
1.4683 0 Td
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2.948 0 Td
(Science)Tj
3.2032 0 Td
(web)Tj
1.8368 0 Td
(server,)Tj
0.83 0.64 0.02 0 k
2.8516 0 Td
(www.matrixscience.com)Tj
0 g
-26.9062 -1.3039 Td
([)Tj
0.83 0.64 0.02 0 k
(34)Tj
0 g
(].)Tj
2.0692 0 Td
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1.0999 0 Td
(addition,)Tj
3.8154 0 Td
(Mascot)Tj
3.1237 0 Td
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(in-house)Tj
3.7247 0 Td
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(consisting)Tj
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1.7801 0 Td
(\(ESTs\))Tj
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(research)Tj
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1.1452 0 Td
(salmon)Tj
3.1351 0 Td
(consortiums)Tj
5.2101 0 Td
([)Tj
0.83 0.64 0.02 0 k
(35)Tj
0 g
(].)Tj
2.0806 0 Td
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1.7801 0 Td
(search)Tj
2.761 0 Td
(parameters)Tj
4.6714 0 Td
(used)Tj
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3.4469 0 Td
(=)Tj
0.7993 0 Td
(Trypsin,)Tj
3.5773 0 Td
(Max.)Tj
2.2394 0 Td
(Missed)Tj
3.05 0 Td
(cleavages)Tj
3.8891 0 Td
(=)Tj
0.7994 0 Td
(1;)Tj
0.9184 0 Td
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2.4321 0 Td
(modifications)Tj
5.7373 0 Td
(=)Tj
0.7994 0 Td
(Carbamidomethyl)Tj
7.5174 0 Td
(\(C\);)Tj
-35.206 -1.2982 Td
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3.5943 0 Td
(modifications)Tj
5.7372 0 Td
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0.7994 0 Td
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4.2803 0 Td
(\(M\);)Tj
2.0183 0 Td
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3.2371 0 Td
(tolerance)Tj
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3.8948 0 Td
[()]TJ
/F8 1 Tf
0.7937 0 Td
(1.5)Tj
1.3946 0 Td
(Da;)Tj
1.6157 0 Td
(MS/MS)Tj
3.2882 0 Td
(tolerance)Tj
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3.8948 0 Td
[()]TJ
/F8 1 Tf
(0.5)Tj
-34.5484 -1.2983 Td
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1.6157 0 Td
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3.2371 0 Td
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(and)Tj
1.7291 0 Td
(3+;)Tj
1.5023 0 Td
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(ESI-TRAP.)Tj
4.6601 0 Td
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-32.326 -1.2983 Td
(EST/contig)Tj
4.7168 0 Td
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3.634 0 Td
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1.0262 0 Td
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0.6519 0 Td
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6.1965 0 Td
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3.5717 0 Td
(search)Tj
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3.0273 0 Td
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2.5399 0 Td
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6.3835 0 Td
(protein)Tj
3.1521 0 Td
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1.0261 0 Td
(identify)Tj
3.2938 0 Td
(the)Tj
1.4684 0 Td
(protein.)Tj
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Q
q
1 j
1 J
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11.9999 0 0 11.9999 200.0125 230.0031 cm
BT
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1 TL
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(RNA)Tj
2.3102 0 Td
(purification)Tj
4.989 0 Td
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(cDNA)Tj
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(synthesis)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 212.9952 cm
BT
/F8 1 Tf
1 TL
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2.313 0 Td
(RNA)Tj
2.262 0 Td
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(extracted)Tj
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(skin)Tj
1.8878 0 Td
(samples)Tj
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(of)Tj
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(10)Tj
1.1735 0 Td
(fish)Tj
1.6611 0 Td
(per)Tj
1.5307 0 Td
(diet)Tj
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(both)Tj
2.0579 0 Td
(Trial#1)Tj
3.1011 0 Td
(and)Tj
1.7291 0 Td
(#2)Tj
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(TRI)Tj
1.7914 0 Td
(reagent)Tj
3.1862 0 Td
(\(Sigma\),)Tj
3.5829 0 Td
(as)Tj
1.0148 0 Td
(instructed)Tj
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1.1848 0 Td
(the)Tj
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