Blar i Nord Open Research Archive på forfatter "Weusthuis, Ruud A."
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Co-production of hydrogen and ethyl acetate in Escherichia coli
Bohnenkamp, Anna C.; Wijffels, Rene Hubertus; Kengen, Servé W. M.; Weusthuis, Ruud A. (Peer reviewed; Journal article, 2021) -
Effect of single and combined expression of Lysophosphatidic Acid Acyltransferase, Glycerol-3-Phosphate Acyltransferase, and Diacylglycerol Acyltransferase on lipid accumulation and composition in Neochloris oleoabundans
Muñoz, Camilo F.; Weusthuis, Ruud A.; D'Adamo, Sarah; Wijffels, Rene Hubertus (Peer reviewed; Journal article, 2019)Microalgal lipids are promising feedstocks for food and biofuels. Since lipid production by microalgae is not yet economically feasible, genetic engineering is becoming a promising strategy to achieve higher lipid accumulation ... -
From Eat to trEat : engineering the mitochondrial Eat1 enzyme for enhanced ethyl acetate production in Escherichia coli
Kruis, Aleksander J.; Bohnenkamp, Anna C.; Nap, Bram; Nielsen, Jochem; Mars, Astrid E.; Wijffels, Rene Hubertus; van der Oost, John; Kengen, Servé W. M.; Weusthuis, Ruud A. (Peer reviewed; Journal article, 2020)Genetic engineering of microorganisms has become a common practice to establish microbial cell factories for a wide range of compounds. Ethyl acetate is an industrial solvent that is used in several applications, mainly ... -
Genetic engineering of microalgae for enhanced lipid production
Muñoz, Camilo F.; Südfeld, Christian; Naduthodi, Mihris I.S.; Weusthuis, Ruud A.; Barbosa, Maria J.; Wijffels, Rene Hubertus; D'Adamo, Sarah (Peer reviewed; Journal article, 2021)Microalgae have the potential to become microbial cell factories for lipid production. Their ability to convert sunlight and CO2 into valuable lipid compounds has attracted interest from cosmetic, biofuel, food and feed ... -
Metabolic engineering of Pseudomonas putida KT2440 for medium-chain-length fatty alcohol and ester production from fatty acids
Lu, Chunzhe; Akwafo, Edward Ofori; Wijffels, Rene Hubertus; Martins dos Santos, Vitor A.P.; Weusthuis, Ruud A. (Peer reviewed; Journal article, 2022) -
Microbial production of medium-chain-length α, ω-diols via two-stage process under mild conditions
Lu, Chunzhe; Leitner, Nina; Wijffels, Rene Hubertus; Martins dos Santos, Vitor A.P.; Weusthuis, Ruud A. (Peer reviewed; Journal article, 2022) -
Multilevel optimisation of anaerobic ethyl acetate production in engineered Escherichia coli
Bohnenkamp, Anna C.; Kruis, Aleksander J.; Mars, Astrid E.; Wijffels, Rene Hubertus; van der Oost, John; Kengen, Servé W. M.; Weusthuis, Ruud A. (Peer reviewed; Journal article, 2020)Background: Ethyl acetate is a widely used industrial solvent that is currently produced by chemical conversions from fossil resources. Several yeast species are able to convert sugars to ethyl acetate under aerobic ... -
Overexpression of delta-12 desaturase in the yeast Schwanniomyces occidentalis enhances the production of linoleic acid
Lamers, Dennis; Visscher, Bram; Weusthuis, Ruud A.; Francke, Christof; Wijffels, Rene Hubertus; Lokman, Christien (Peer reviewed; Journal article, 2019)The oleaginous yeast Schwanniomyces occidentalis was previously isolated because of its excellent suitability to convert lignocellulosic hydrolysates into triacyl glycerides: it is able to use a broad range of sugars and ... -
Stable transformation of the green algae Acutodesmus obliquus and Neochloris oleoabundans based on E. coli conjugation
Muñoz, Camilo F.; Sturme, Mark H.J.; D'Adamo, Sarah; Weusthuis, Ruud A.; Wijffels, Rene Hubertus (Peer reviewed; Journal article, 2019)Microalgae are an ideal platform for the production of high-value chemicals, nutritional products and biofuels. Genetic engineering could speed up the development of microalgae derived products and reduce the overall ... -
When metabolic prowess is too much of a good thing : how carbon catabolite repression and metabolic versatility impede production of esterified α,ω-diols in Pseudomonas putida KT2440
Lu, Chunzhe; Batianis, Christos; Akwafo, Edward Ofori; Wijffels, Rene Hubertus; Martins dos Santos, Vitor A.P.; Weusthuis, Ruud A. (Peer reviewed; Journal article, 2021)Background: Medium-chain-length α,ω-diols (mcl-diols) are important building blocks in polymer production. Recently, microbial mcl-diol production from alkanes was achieved in E. coli (albeit at low rates) using the alkane ...