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dc.contributor.authorMeier, Sonnich
dc.contributor.authorKarlsen, Ørjan
dc.contributor.authorLe Goff, Jérémie
dc.contributor.authorSørensen, Lisbet
dc.contributor.authorSørhus, Elin
dc.contributor.authorPampanin, Daniela Maria
dc.contributor.authorDonald, Carey E.
dc.contributor.authorFjelldal, Per Gunnar
dc.contributor.authorDunaevskaya, Evgenia
dc.contributor.authorRomano, Marta
dc.contributor.authorCaliani, Ilaria
dc.contributor.authorCasini, Silvia
dc.contributor.authorBogevik, Andre S.
dc.contributor.authorOlsvik, Pål Asgeir
dc.contributor.authorMyers, Mark
dc.contributor.authorGrøsvik, Bjørn Einar
dc.identifier.citationMeier, S., Karlsen, Ø., Le Goff, J., Sørensen, L., Sørhus, E., Pampanin, D. M. ... Grøsvik, B. E. (2020). DNA damage and health effects in juvenile haddock (Melanogrammus aeglefinus) exposed to PAHs associated with oil-polluted sediment or produced water. PLoS ONE, 15(10). doi:en_US
dc.description.abstractThe research objective was to study the presence of DNA damages in haddock exposed to petrogenic or pyrogenic polyaromatic hydrocarbons (PAHs) from different sources: 1) extracts of oil produced water (PW), dominated by 2-ring PAHs; 2) distillation fractions of crude oil (representing oil-based drilling mud), dominated by 3-ring PAHs; 3) heavy pyrogenic PAHs, mixture of 4/5/6-ring PAHs. The biological effect of the different PAH sources was studied by feeding juvenile haddock with low doses of PAHs (0.3–0.7 mg PAH/kg fish/day) for two months, followed by a two-months recovery. In addition to the oral exposure, a group of fish was exposed to 12 single compounds of PAHs (4/5/6-ring) via intraperitoneal injection. The main endpoint was the analysis of hepatic and intestinal DNA adducts. In addition, PAH burden in liver, bile metabolites, gene and protein expression of CYP1A, GST activity, lipid peroxidation, skeletal deformities and histopathology of livers were evaluated. Juvenile haddock responded quickly to both intraperitoneal injection and oral exposure of 4/5/6-ring PAHs. High levels of DNA adducts were detected in livers three days after the dose of the single compound exposure. Fish had also high levels of DNA adducts in liver after being fed with extracts dominated by 2-ring PAHs (a PW exposure scenario) and 3-ring PAHs (simulating an oil exposure scenario). Elevated levels of DNA adducts were observed in the liver of all exposed groups after the 2 months of recovery. High levels of DNA adduct were found also in the intestines of individuals exposed to oil or heavy PAHs, but not in the PW or control groups. This suggests that the intestinal barrier is very important for detoxification of orally exposures of PAHs.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleDNA damage and health effects in juvenile haddock (Melanogrammus aeglefinus) exposed to PAHs associated with oil-polluted sediment or produced wateren_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© 2020 The Author(s)en_US
dc.subject.nsiVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økotoksikologi: 489en_US
dc.source.journalPLOS ONEen_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal