dc.contributor.author | Beirão, José | |
dc.contributor.author | Flengstad, Stian | |
dc.contributor.author | Babiak, Igor | |
dc.date.accessioned | 2021-03-10T08:05:33Z | |
dc.date.available | 2021-03-10T08:05:33Z | |
dc.date.created | 2021-02-15T10:46:27Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Beirão, J., Flengstad, S. & Babiak, I. (2020). Spotted wolffish (Anarhichas minor) sperm cryopreservation in 5-mL cryovials. Fish Physiology & Biochemistry. doi: | en_US |
dc.identifier.issn | 1573-5168 | |
dc.identifier.uri | https://hdl.handle.net/11250/2732535 | |
dc.description.abstract | In spotted wolffish Anarhichas minor aquaculture, cryopreservation is used to secure sperm availability throughout the entire spawning season. Under current protocols, sperm is cryopreserved in 0.5-mL straws. This implies thawing a considerable number of straws for insemination with cryopreserved sperm. In this work, we scale up the spotted wolffish sperm cryopreservation procedure through the development of a protocol for sperm cryopreservation in 5-mL cryovials. Different freezing (distances from the liquid nitrogen surface) and thawing rates were tested. The best results were obtained with cryovials frozen at a distance of 1.5 cm from the liquid nitrogen surface and thawed either at 15 or 10 °C for 4 and 6 min, respectively. Under these conditions, similar percentage of motile cells, sperm velocity and percentage of viable cells were obtained in comparison with the sperm cryopreserved in the traditional 0.5-mL straws. This protocol will facilitate the process of insemination with cryopreserved sperm in the spotted wolffish hatcheries. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Springer | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Spotted wolffish (Anarhichas minor) sperm cryopreservation in 5-mL cryovials | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | © 2020 The Author(s) | en_US |
dc.subject.nsi | VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Akvakultur: 922 | en_US |
dc.subject.nsi | VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Embryologi: 482 | en_US |
dc.source.pagenumber | 5 | en_US |
dc.source.journal | Fish Physiology & Biochemistry | en_US |
dc.identifier.doi | 10.1007/s10695-020-00837-1 | |
dc.identifier.cristin | 1889759 | |
dc.relation.project | MABIT: AF0078 | en_US |
dc.description.localcode | Unit License Agreement | en_US |