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dc.contributor.authorAbihssira Garcia, Isabel Sofia
dc.contributor.authorPark, Youngjin
dc.contributor.authorViswanath, Kiron
dc.contributor.authorOlsvik, Pål Asgeir
dc.date.accessioned2021-03-12T14:18:51Z
dc.date.available2021-03-12T14:18:51Z
dc.date.created2020-11-20T13:17:42Z
dc.date.issued2020
dc.identifier.citationAbihssira-Garcia, I. S., Park, Y., Viswanath, K. & Olsvik, P. A. (2020). Fluorescent Microplastic Uptake by Immune Cells of Atlantic Salmon (Salmo salar L.). Frontiers in Environmental Science, 8: 560206. doi:en_US
dc.identifier.issn2296-665X
dc.identifier.urihttps://hdl.handle.net/11250/2733204
dc.description.abstractThe ubiquitous presence of microplastics and their marine ecotoxicity are major public concerns. Microplastics are ingested accidentally by the marine fauna or are taken up indirectly through the food chain. These particles can accumulate in cells and tissues and affect the normal biological functions of organisms, including their defense mechanisms. There is limited information available about the response of immune cells to microplastics; the degree of uptake by the cells, the response of different organs or the impact of environmental concentrations of microplastic are matters that remain unclear. Moreover, very little is known about the toxicity of different polymer types. This study aimed to shed light on the physical impact of small microplastics (1–5 μm) on cells from Atlantic salmon. Immune cells from intestine, blood, and head kidney were exposed to green fluorescent polyethylene microplastic (PE-MP), yellow fluorescent polystyrene microplastic (PS-MP) and both. High (50 mg/L), medium (5 mg/L), and low (0.05 mg/L) concentrations were tested for 1, 24, 48, and 72 h to study cell mortality and microplastic uptake. Quantitative data of microplastic uptake by fish immune cells were obtained for the first time by imaging flow cytometry. Salmon immune cells showed a relatively low ability to phagocytose microplastics. Less than 6% of the cells ingested the particles after 48 h of exposure to high concentrations. Cells also phagocytosed microplastics at low concentrations although at low rates (<0.1%). PE-MPs was phagocytosed by higher percentage of cells compared to PS-MPs and the former bioaccumulated in time while the latter decreased over time. However, each cell generally phagocytosed more PS-MPs particles than PE-MPs. Cells from different tissues showed different responses to the microplastic polymers. In conclusion, this study shows that immune cells of Atlantic salmon can phagocytose microplastics, and the impact is dependent on the microplastic type. PE-MPs, the most abundant polymer in the oceans and a widely used plastic in salmon aquaculture, was more easily taken up than PS-MPs. Furthermore, the study demonstrates how imaging flow cytometry can be applied in microplastics research.en_US
dc.language.isoengen_US
dc.publisherFrontiersen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleFluorescent Microplastic Uptake by Immune Cells of Atlantic Salmon (Salmo salar L.)en_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.rights.holder© 2020 The Author(s)en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Økologi: 488en_US
dc.source.volume8en_US
dc.source.journalFrontiers in Environmental Scienceen_US
dc.identifier.doi10.3389/fenvs.2020.560206
dc.identifier.cristin1850413
dc.description.localcodeUnit Licence Agreementen_US
dc.source.articlenumber560206en_US


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal