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dc.contributor.authorNagasawa, Kazue
dc.contributor.authorPresslauer, Christopher
dc.contributor.authorKirtiklis, Lech
dc.contributor.authorBabiak, Igor
dc.contributor.authorFernandes, Jorge M.O.
dc.date.accessioned2015-03-14T07:20:26Z
dc.date.accessioned2015-03-16T10:05:35Z
dc.date.available2015-03-14T07:20:26Z
dc.date.available2015-03-16T10:05:35Z
dc.date.issued2014
dc.identifier.citationNagasawa, K., Presslauer, C., Kirtiklis, L., Babiak, I. & Fernandes, J.M.O. (2014). Sexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cycle. Journal of Molecular Endocrinology , 52(3), 357-371. doi:nb_NO
dc.identifier.issn1479-6813
dc.identifier.urihttp://hdl.handle.net/11250/279299
dc.descriptionAuthor's accepted version (post-print).nb_NO
dc.descriptionThis manuscript has been accepted for publication in Journal of Molecular Endocrinology, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, BioScientifica accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at http://dx.doi.org/10.1530/JME-13-0187, 2014.
dc.description.abstractThe role of sex steroid regulation in gonadal maturation is a very complex process that is far from being fully understood. Hence, we have investigated seasonal changes in gonadal expression of estrogen receptors (ERs) in Atlantic cod (Gadus morhua L.), a batch spawner, throughout annual reproductive cycle. Three nuclear ER partial cDNA sequences (esr1, esr2a and esr2b) were cloned and all esr transcripts were detected mainly in liver and gonads of either sex. In situ hybridization of esrs along with germ cell (vasa) and gonadal somatic cell markers (gsdf, 3β-hsd and amh for testicular, or gsdf for ovarian somatic cells) showed that in testis all three esrs were preferentially localized within interstitial fibroblasts composed of immature and mature Leydig cells, whereas in ovary they were differentially expressed in both follicular cells and oocytes. Quantitative real-time PCR analysis revealed a sexually dimorphic expression pattern of the three esr paralogues in testis and ovary. A significant increase in esr2a expression was identified in testis and esr2b in ovary, whereas esr1 transcripts were elevated in both testis and ovary at February and March prior to the spawning period. The localization and sexually dimorphic expression of esr genes in gonads suggest a direct function of estrogen via ERs in gonadal somatic cell growth and differentiation for Leydig cell in testis and follicular cells in ovary throughout the annual reproductive cycle in Atlantic cod.nb_NO
dc.language.isoengnb_NO
dc.publisherBioScientificanb_NO
dc.titleSexually dimorphic transcription of estrogen receptors in cod gonads throughout a reproductive cyclenb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.date.updated2015-03-14T07:20:26Z
dc.subject.nsiVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923nb_NO
dc.source.pagenumber357-371nb_NO
dc.source.volume52nb_NO
dc.source.journalJournal of Molecular Endocrinologynb_NO
dc.source.issue3nb_NO
dc.identifier.doi10.1530/JME-13-0187
dc.identifier.cristin1129249


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