Browsing Nord Open Research Archive by Author "Martens, Dirk E."

Automation of high CHO cell density seed intensification via online control of the cell specific perfusion rate and its impact on the N-stage inoculum quality

Schulze, Markus; Lemke, Johannes; Pollard, David; Wijffels, Rene Hubertus; Matuszczyk, Jens; Martens, Dirk E. (Peer reviewed; Journal article, 2021)

Bioengineering bacterial outer membrane vesicles as vaccine platform

Gerritzen, Matthias J.H.; Martens, Dirk E.; Wijffels, Rene Hubertus; van der Pol, Leo; Stork, Michiel (Journal article; Peer reviewed, 2017)

Breakthrough in marine invertebrate cell culture : Sponge cells divide rapidly in improved nutrient medium

Conkling, Megan; Hesp, Kylie; Munroe, Stephanie; Sandoval, Kenneth; Martens, Dirk E.; Sipkema, Detmer; Wijffels, Rene Hubertus; Pomponi, Shirley A. (Peer reviewed; Journal article, 2019)

Sponges (Phylum Porifera) are among the oldest Metazoa and considered critical to understanding animal evolution and development. They are also the most prolific source of marine-derived chemicals with pharmaceutical ...

Continuous production of Neisseria meningitidis outer membrane vesicles

Gerritzen, Matthias J.H.; Stangowez, Lilli; van de Waterbeemd, Bas; Martens, Dirk E.; Wijffels, Rene Hubertus; Stork, Michiel (Peer reviewed; Journal article, 2019)

Outer membrane vesicles (OMVs) are nanoparticles secreted by Gram-negative bacteria that can be used for diverse biotechnological applications. Interesting applications have been developed, where OMVs are the basis of drug ...

CRISPR/Cas12a-mediated gene editing in Geodia barretti sponge cell culture

Hesp, Kylie; Alvarez, John L. Flores; Alexandru, Ana-Maria; van der Linden, Jip; Martens, Dirk E.; Wijffels, Rene Hubertus; Pomponi, Shirley A. (Peer reviewed; Journal article, 2020)

Sponges and their associated microorganisms are the most prolific source of marine natural products, and many attempts have been made at creating a marine sponge cell line to produce these products efficiently. However, ...

Dynamics of triacylglycerol and EPA production in Phaeodactylum tricornutum under nitrogen starvation at different light intensities

Remmers, Ilse M.; Martens, Dirk E.; Wijffels, Rene Hubertus; Lamers, Packo P. (Journal article; Peer reviewed, 2017)

Evaluation of diurnal responses of Tetradesmus obliquus under nitrogen limitation

Leon-Saiki, G. Mitsue; Carreres, Benoit M.; Remmers, Ilse M.; Wijffels, Rene Hubertus; dos Santos, Vitor A.P. Martins; van der Veen, Douwe; Schaap, Peter J.; Suarez-Diez, Maria; Martens, Dirk E. (Peer reviewed; Journal article, 2020)

Tetradesmus obliquus is an oleaginous microalga with high potential for triacylglycerol production. We characterized the biochemical composition and the transcriptional landscape of T. obliquus wild-type and the starchless ...

Gene silencing of stearoyl-ACP desaturase enhances the stearic acid content in Chlamydomonas reinhardtii

de Jaeger, Lenny; Springer, Jan; Wolbert, Emil J.H.; Martens, Dirk E.; Eggink, Gerrit; Wijffels, Rene Hubertus (Journal article; Peer reviewed, 2017)

High dissolved oxygen tension triggers outer membrane vesicle formation by Neisseria meningitidis

Gerritzen, Matthias J.H.; Maas, Ronald H. W.; Van Den Ijssel, Jan; Van Keulen, Lonneke; Martens, Dirk E.; Wijffels, Rene Hubertus; Stork, Michiel (Journal article; Peer reviewed, 2018)

High throughput nanoparticle tracking analysis for monitoring outer membrane vesicle production

Gerritzen, Matthias J.H.; Martens, Dirk E.; Wijffels, Rene Hubertus; Stork, Michiel (Journal article; Peer reviewed, 2017)

Metabolic characterization of a CHO cell size increase phase in fed-batch cultures

Pan, Xiao; Dalm, Ciska; Wijffels, Rene Hubertus; Martens, Dirk E. (Journal article; Peer reviewed, 2017)

Neochloris oleoabundans is worth its salt : Transcriptomic analysis under salt and nitrogen stress

de Jaeger, Lenny; Carreres, Benoit M.; Springer, Jan; Schaap, Peter J.; Eggink, Gerrit; Martins Dos Santos, Vitor A. P.; Wijffels, Rene Hubertus; Martens, Dirk E. (Journal article; Peer reviewed, 2018)

Orchestration of transcriptome, proteome and metabolome in the diatom Phaeodactylum tricornutum during nitrogen limitation

Remmers, Ilse M.; D'Adamo, Sarah; Martens, Dirk E.; de Vos, Ric C. H.; Mumm, Roland; America, Antoine H. P.; Cordewener, Jan H. G.; Bakker, Linda V.; Peters, Sander; Wijffels, Rene Hubertus; Lamers, Packo P. (Journal article; Peer reviewed, 2018)

Rapid intensification of an established CHO cell fed-batch process

Schulze, Markus; Niemann, Julia; Wijffels, Rene Hubertus; Matuszczyk, Jens; Martens, Dirk E. (Peer reviewed; Journal article, 2021)

Currently, the mammalian biomanufacturing industry explores process intensification (PI) to meet upcoming demands of biotherapeutics while keeping production flexible but, more importantly, as economic as possible. However, ...

Selection of chemically defined media for CHO cell fed-batch culture processes

Pan, Xiao; Streefland, Mathieu; Dalm, Ciska; Wijffels, Rene Hubertus; Martens, Dirk E. (Journal article; Peer reviewed, 2016)

Spontaneously released Neisseria meningitidis outer membrane vesicles as vaccine platform : production and purification

Gerritzen, Matthias J.H.; Salverda, Merijn LM; Martens, Dirk E.; Wijffels, Rene Hubertus; Stork, Michiel (Peer reviewed; Journal article, 2019)

Outer membrane vesicles (OMVs) are nanoparticles produced by Gram-negative bacteria that can be used as vaccines. The application of OMVs as vaccine component can be expanded by expressing heterologous antigens on OMVs, ...

Sulfate depletion triggers overproduction of phospholipids and the release of outer membrane vesicles by Neisseria meningitidis

Gerritzen, Matthias J.H.; Martens, Dirk E.; Uittenbogaard, Joost P.; Wijffels, Rene Hubertus; Stork, Michiel (Peer reviewed; Journal article, 2019)

Outer membrane vesicles (OMVs) produced by bacteria are interesting vaccine candidates. OMVs are nanoparticles that contain many immunogenic components, are self-adjuvating, and non-replicative. Despite recent insights in ...