Nord Open Research Archive: Differential expression patterns of conserved miRNAs and isomiRs during Atlantic halibut development

Bizuayehu, Teshome T.; Lanes, Carlos Fredrico Ceccon; Furmanek, Tomasz; Karlsen, Bård Ove; Fernandes, Jorge M.O.; Johansen, Steinar Daae; Babiak, Igor

Journal article, Peer reviewed

View/Open

Babiak.pdf (490.5Kb)

Additional file 1: Read counts for 8 developmental stages of Atlantic halibut. The total number of raw sequence reads obtained from SOLiD sequencing and the number of usable reads for each developmental stage is given. (34.28Kb)

Additional file 2: Size distribution of mapped reads in Atlantic halibut deep sequencing data. Reads mapped to different databases: A) mitochondrial transcripts, rRNA, tRNA, other non-coding RNA; B) miRBase 16 and C) Atlantic halibut ESTs. The size distribution of mapped reads for each database is given. (29.67Kb)

Additional file 3: Identified conserved miRNAs. All identified conserved Atlantic halibut miRNAs along with miRBase name, nucleotide sequences and read counts for each investigated developmental stages are given. Lower case letters indicate mismatches comparing to references. (25.63Kb)

Additional file 4: miRNA diversity during early development of Atlantic halibut. The number of different types of miRNAs identified during 8 stages of Atlantic halibut development; increasing as the development progressed. (9.166Kb)

Additional file 5: IsomiR occurrence during early development of Atlantic halibut. All miRNAs having ≥ 0.01% truncation/s or addition/s in 5' and/or 3' ends are listed. The percentage of both template and non-template nucleotide changes (A, U, C, and G) are shown as percentage of total reads of a given miRNA. (48Kb)

Additional file 6: Cloned miRNA sequences and their frequency. Indentified conserved miRNAs using cloning technique and their frequencies. Nucleotide mismatches (green), additions (blue) or modifications (red) are found in some miRNAs. (29.5Kb)

URI

http://hdl.handle.net/11250/141130

Date

2012

Metadata

Show full item record

Collections

Artikler, rapporter og annet (FBA) [806]

Original version

Bizuayehu, T.T., Lanes, C.F.C., Furmanek, T., Karlsen, B.O., Fernandes, J.M.O., Johansen, S.D. & Babiak, I. (2012). Differential expression patterns of conserved miRNAs and isomiRs during Atlantic halibut development. BMC Genomics, 13: 11. doi: 10.1186/1471-2164-13-11

Publisher

BioMed Central

Journal

BMC Genomics

Copyright

© 2012, The Author(s)