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Synergistic activity of Atlantic cod (Gadus morhua L.) piscidin with other antibiotic agents

Juntarut, Pongsaton
Master thesis
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URI
http://hdl.handle.net/11250/141197
Date
2010
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  • Masteroppgaver i havbruk og akvakultur [53]
Abstract
Disease management is crucial in the aquaculture industry and to date it

involves the usage of antibiotics to treat and prevent disease, especially bacterial

infections. However, there is a growing concern over the problems caused by

conventional antibiotics in aquatic and terrestrial environments (eg, bacteria resistant

to multiple antibiotics). Therefore, it is crucial to reduce the usage of conventional

antibiotics or find alternative novel antibacterial agents to manage bacterial diseases

and antibiotic-resistant bacteria. Antimicrobial peptides are natural antibiotics

produced from multicellular organisms and have the ability to kill bacteria and to

synergise with other antibiotics. In this study I have analysed the in-vitro antibacterial

activity of synthetic Atlantic cod piscidin and its ability to permeabilize the bacterial

membrane. Moreover, I have investigated the antimicrobial properties of synthetic cod

piscidin in combination with several conventional antibiotics, including oxolinic acid,

oxytetracycline hydrochloride, and sulfadiazine/trimethoprim, which are amongst the

most important groups of antibiotics for prevention of bacterial diseases in

aquaculture. Five test bacteria, including two Gram-positive bacteria (Micrococcus

lysodeikticus and Planococcus citreus) and three Gram-negative bacteria (Yersinia

ruckeri VI 3629, Vibrio anguillarum VI-F-258-3 and Aeromonas salmonicida NCIMB

1102) were tested for synergistic activity of peptide-antibiotic combination.

Antibacterial activity results showed moderate synergism i) between oxolinic acid and

synthetic cod piscidin against V. anguillarum VI-F-258-3; ii) combined

oxytetracycline hydrochloride and synthetic cod piscidin against V. anguillarum VI-F-

258-3 and A. salmonicida NCIMB 1102; iii) and combined sulfadiazine/trimethoprim

and synthetic cod piscidin against M. lysodeikticus, A. salmonicida NCIMB 1102, Y.

ruckeri VI 3629. In contrast, no synergistic activity of either test antibiotics with

synthetic cod piscidin was found against P. citreus. The data show that synthetic cod

piscidin can reduce the concentration of conventional antibiotics required to inhibit

bacterial growth of fish pathogenic bacteria, namely Y. ruckeri VI 3629, V.

anguillarum VI-F-258-3 and A. salmonicida NCIMB 1102. Flow cytometry analysis

revealed that this peptide could form stable pores in the bacterial membrane, which

might be its main mechanism of action. These properties of synthetic cod piscidin

highlight its potential an novel antibacterial agent that in a not so distant future may be

used in disease control management in commercial aquaculture systems.
Description
Masteroppgave i havbruk - Høgskolen i Bodø, 2010
Publisher
Høgskolen i Bodø

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