Blar i Nord Open Research Archive på forfatter "Wijffels, Rene Hubertus"
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Continuous versus batch production of lipids in the microalgae Acutodesmus obliquus
Remmers, Ilse M.; Hidalgo-Ulloa, A.; Brandt, B.P.; Evers, Wendy A.C.; Wijffels, Rene Hubertus; Lamers, Packo P. (Journal article; Peer reviewed, 2017) -
CRISPR/Cas12a-mediated gene editing in Geodia barretti sponge cell culture
Hesp, Kylie; Alvarez, John L. Flores; Alexandru, Ana-Maria; van der Linden, Jip; Martens, Dirk E.; Wijffels, Rene Hubertus; Pomponi, Shirley A. (Peer reviewed; Journal article, 2020)Sponges and their associated microorganisms are the most prolific source of marine natural products, and many attempts have been made at creating a marine sponge cell line to produce these products efficiently. However, ... -
Cultivation of shear stress sensitive and tolerant microalgalspecies in a tubular photobioreactor equipped with a centrifugal pump
Michels, Michiel H.A.; Van der Goot, Atze Jan; Vermuë, Marian H.; Wijffels, Rene Hubertus (Journal article; Peer reviewed, 2015) -
Cyanobacterial growth and cyanophycin production with urea and ammonium as nitrogen source
Canizales, Samuel; Sliwszcinka, M.; Russo, A.; Bentvelzen, S.; Temmink, H.; Verschoor, A. M.; Wijffels, Rene Hubertus; Janssen, Maarten (Peer reviewed; Journal article, 2021) -
Design of value chains for microalgal biorefinery at industrial scale : process integration and techno-economic analysis
Slegers, Petronella M.; Olivieri, Giuseppe; Breitmayer, Elke; Sijtsma, Lolke; Eppink, Michel H.M.; Wijffels, Rene Hubertus; Reith, Johannes H. (Peer reviewed; Journal article, 2020)The objective of this work was to identify industrial scenarios for the most promising microalgal biorefinery value chains on the basis of product selection, yields, and techno-economic performance, using biological ... -
Developing microalgal oil production for an outdoor photobioreactor
Norsker, Niels Henrik; Cuaresma, Maria; Uronen, Pauliina; Barbosa, Maria J.; Wijffels, Rene Hubertus (Peer reviewed; Journal article, 2021)In this paper the preparations are described to develop a production of oil rich microalgal biomass under south European conditions. Ten microalgal species were compared in shake flasks in an incubator for potential for ... -
Different co-occurring bacteria enhance or decrease the growth of the microalga Nannochloropsis sp. CCAP211/78
Lian, Jie; Schimmel, Patrick; Sanchez-Garcia, Selene; Wijffels, Rene Hubertus; Smidt, Hauke; Sipkema, Detmer (Peer reviewed; Journal article, 2021) -
Diversity of tryptophan halogenases in sponges of the genus Aplysina
Gutleben, Johanna; Koehorst, Jasper J; McPherson, Kyle; Pomponi, Shirley; Wijffels, Rene Hubertus; Smidt, Hauke; Sipkema, Detmer (Peer reviewed; Journal article, 2019)Marine sponges are a prolific source of novel enzymes with promising biotechnological potential. Especially halogenases, which are key enzymes in the biosynthesis of brominated and chlorinated secondary metabolites, possess ... -
Dosage effect of cationic polymers on the flocculation efficiency of the marine microalga Neochloris oleoabundans
't Lam, G.P.; Zegeye, E.K.; Vermuë, M.H.; Kleinegris, D.M.M.; Eppink, M.H.M.; Wijffels, Rene Hubertus; Olivieri, G. (Journal article; Peer reviewed, 2015) -
Doubling of Microalgae Productivity by Oxygen Balanced Mixotrophy
Abiusi, Fabian; Wijffels, Rene Hubertus; Janssen, Marcel (Peer reviewed; Journal article, 2020)Microalgae productivity was doubled by designing an innovative mixotrophic cultivation strategy that does not require gas–liquid transfer of oxygen or carbon dioxide. Chlorella sorokiniana SAG 211/8K was cultivated under ... -
Dynamics of triacylglycerol and EPA production in Phaeodactylum tricornutum under nitrogen starvation at different light intensities
Remmers, Ilse M.; Martens, Dirk E.; Wijffels, Rene Hubertus; Lamers, Packo P. (Journal article; Peer reviewed, 2017) -
Effect of ammonium formate washing on the elemental composition determination in Nannochloropsis oceanica
Guimarães, Bárbara O.; Gremmen, Pieter; Wijffels, Rene Hubertus; Barbosa, Maria J.; D'Adamo, Sarah (Peer reviewed; Journal article, 2021) -
Effect of initial biomass-specific photon supply rate on fatty acid accumulation in nitrogen depleted Nannochloropsis gaditana under simulated outdoor light conditions
Janssen, Jorijn H.; Driessen, Jasper L. S. P.; Lamers, Packo P.; Wijffels, Rene Hubertus; Barbosa, Maria (Journal article; Peer reviewed, 2018) -
Effect of removal of bacteria on the biomass and extracellular carbohydrate productivity of Botryococcus braunii
Gouveia, João Diogo; Moers, Antoine; Griekspoor, Yvonne; van den Broek, Lambertus A.M.; Springer, Jan; Sijtsma, Lolke; Sipkema, Detmer; Wijffels, Rene Hubertus; Barbosa, Maria (Peer reviewed; Journal article, 2019)Botryococcus braunii CCALA778 is a green microalga that can produce large amounts of extracellular carbohydrates and therefore is a potential host for industrial applications such as materials, food and pharmaceutical ... -
Effect of single and combined expression of Lysophosphatidic Acid Acyltransferase, Glycerol-3-Phosphate Acyltransferase, and Diacylglycerol Acyltransferase on lipid accumulation and composition in Neochloris oleoabundans
Muñoz, Camilo F.; Weusthuis, Ruud A.; D'Adamo, Sarah; Wijffels, Rene Hubertus (Peer reviewed; Journal article, 2019)Microalgal lipids are promising feedstocks for food and biofuels. Since lipid production by microalgae is not yet economically feasible, genetic engineering is becoming a promising strategy to achieve higher lipid accumulation ... -
Efficient Fractionation of Lipids in a Multiproduct Microalgal Biorefinery by Polymers and Ionic Liquid-Based Aqueous Two-Phase Systems
Ruiz, Catalina A. Suarez; Cabau-Peinado, Oriol; van den Berg, Corjan; Wijffels, Rene Hubertus; Eppink, Michel H.M. (Peer reviewed; Journal article, 2021)For a multiproduct microalgal biorefinery, most of the cell components should be extracted and fractionated. This work investigates the fractionation of lipids from other microalgal components (pigments, proteins, ... -
Energy efficient bead milling of microalgae : Effect of bead size on disintegration and release of proteins and carbohydrates
Postma, P.R.; Suarez-Garcia, E.; Safi, C.; Yonathan, K.; Olivieri, G.; Barbosa, M.J.; Wijffels, Rene Hubertus; Eppink, M.H.M. (Journal article; Peer reviewed, 2016) -
Eutectic solvents with tuneable hydrophobicity : lipid dissolution and recovery
Lo, Calvin; Semerel, Jeltzlin; van den Berg, Corjan; Wijffels, Rene Hubertus; Eppink, Michel H.M. (Peer reviewed; Journal article, 2021)Despite the promising advantages of eutectic solvents, the application of these solvents as an extraction solvent is still limited due to the challenging product recovery. Previously, it was reported that lipids could be ... -
Evaluation of diurnal responses of Tetradesmus obliquus under nitrogen limitation
Leon-Saiki, G. Mitsue; Carreres, Benoit M.; Remmers, Ilse M.; Wijffels, Rene Hubertus; dos Santos, Vitor A.P. Martins; van der Veen, Douwe; Schaap, Peter J.; Suarez-Diez, Maria; Martens, Dirk E. (Peer reviewed; Journal article, 2020)Tetradesmus obliquus is an oleaginous microalga with high potential for triacylglycerol production. We characterized the biochemical composition and the transcriptional landscape of T. obliquus wild-type and the starchless ... -
Flashing light does not improve photosynthetic performance and growth of green microalgae
Schulze, Peter Simon Claus; Brindley, Celeste; Fernández, José M.; Rautenberger, Ralf; Pereira, Hugo; Wijffels, Rene Hubertus; Kiron, Viswanath (Peer reviewed; Journal article, 2020)Light attenuation in photobioreactors is a major bottleneck in microalgal production. A possible strategy for artificial light-based microalgal production to deliver light deep inside the culture is through the periodical ...