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dc.contributor.authorLazado, Carlo Cabacang
dc.contributor.authorKumaratunga, Hiruni
dc.contributor.authorNagasawa, Kazue
dc.contributor.authorBabiak, Igor
dc.contributor.authorGiannetto, Alessia
dc.contributor.authorFernandes, Jorge M.O.
dc.identifier.citationLazado, C.C., Kumaratunga, H.P.S., Nagasawa, K., Babiak, I., Giannetto, A., & Fernandes, J.M.O. (2014). Daily Rhythmicity of Clock Gene Transcripts in Atlantic Cod Fast Skeletal Muscle. PLoS One, 9(6): e99172. doi:nb_NO
dc.description.abstractThe classical notion of a centralized clock that governs circadian rhythmicity has been challenged with the discovery of peripheral oscillators that enable organisms to cope with daily changes in their environment. The present study aimed to identify the molecular clock components in Atlantic cod (Gadus morhua) and to investigate their daily gene expression in fast skeletal muscle. Atlantic cod clock genes were closely related to their orthologs in teleosts and tetrapods. Synteny was conserved to varying degrees in the majority of the 18 clock genes examined. In particular, aryl hydrocarbon receptor nuclear translocator-like 2 (arntl2), RAR-related orphan receptor A (rora) and timeless (tim) displayed high degrees of conservation. Expression profiling during the early ontogenesis revealed that some transcripts were maternally transferred, namely arntl2,cryptochrome 1b and 2 (cry1b and cry2), and period 2a and 2b(per2a and per2b). Most clock genes were ubiquitously expressed in various tissues, suggesting the possible existence of multiple peripheral clock systems in Atlantic cod. In particular, they were all detected in fast skeletal muscle, with the exception of neuronal PAS (Per-Arnt-Single-minded)domain-containing protein (npas1) and rora. Rhythmicity analysis revealed 8 clock genes with daily rhythmic expression, namely arntl2, circadian locomotor output cycles kaput (clock), npas2, cry2, cry3 per2a, nuclear receptor subfamily 1, group D, member 1 (nr1d1), and nr1d2a. Transcript levels of the myogenic genes myogenic factor 5 (myf5) and muscleblind-like 1(mbnl1) strongly correlated with clock gene expression. This is the first study to unravel the molecular components of peripheral clocks in Atlantic cod. Taken together, our data suggest that the putative clock system in fast skeletal muscle of Atlantic cod has regulatory implications on muscle physiology, particularly in the expression of genes related to myogenesis.nb_NO
dc.publisherPublic Library of Sciencenb_NO
dc.rightsNavngivelse 3.0 Norge*
dc.titleDaily Rhythmicity of Clock Gene Transcripts in Atlantic Cod Fast Skeletal Musclenb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.rights.holder© 2014, The Author(s)
dc.subject.nsiVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Resource biology: 921
dc.subject.nsiVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922
dc.source.journalPLoS ONEnb_NO
dc.relation.projectNorges forskningsråd: 190350nb_NO

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Navngivelse 3.0 Norge
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