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dc.contributor.authorDesai, Rupali K.
dc.contributor.authorFernandez, Maria Salvador
dc.contributor.authorWijffels, Rene Hubertus
dc.contributor.authorEppink, Michel H.M.
dc.identifier.citationDesai, R. K., Fernandez, M. S., Wijffels, R. H. & Eppink, M. H. M. (2019). Mild fractionation of hydrophilic and hydrophobic components from Neochloris oleoabundans using ionic liquids. Frontiers in Bioengineering and Biotechnology, 7: 284. doi:en_US
dc.description.abstractMicroalgae are a promising source for proteins, lipids, and carbohydrates for the food/feed and biofuel industry. To make microalgae production economically feasible, it is necessary to optimally use all produced compounds keeping full functionality. Therefore, biorefining of microalgae is the key to lower the cost of algal products using mild and effective processing techniques. In this article, we have tested the feasibility of aqueous solutions of imidazolium and phosponium ionic liquids to selectively milk the hydrophobic lipids from Neochloris oleoabundans biomass out of intact cells and recover after cell disruption the hydrophilic fraction containing proteins and carbohydrates. The results showed that the ionic liquid tributylmethylphosphonium methylsulfate (TBP SO4; Cyphos 108) is able to permeabilize fresh intact cells of N. oleoabundans for extracting 68% of total lipids out of the cells, whereas, after cell disruption, 80% of total proteins, and 77% of total carbohydrates could be obtained in aqueous buffers. This concept kept the recovered proteins in their native form without interacting with the ionic liquids that will denature the proteins. Selective biorefinery of different components from microalgae using ionic liquid TBP SO4 explains the novelty of this concept.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleMild fractionation of hydrophilic and hydrophobic components from Neochloris oleoabundans using ionic liquidsen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© The Author(s)en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470en_US
dc.source.journalFrontiers in Bioengineering and Biotechnologyen_US
dc.relation.projectTechnology Foundation STW: 11410en_US

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