dc.contributor.author | Lamers, Dennis | |
dc.contributor.author | Visscher, Bram | |
dc.contributor.author | Weusthuis, Ruud A. | |
dc.contributor.author | Francke, Christof | |
dc.contributor.author | Wijffels, Rene Hubertus | |
dc.contributor.author | Lokman, Christien | |
dc.date.accessioned | 2020-06-22T12:59:27Z | |
dc.date.available | 2020-06-22T12:59:27Z | |
dc.date.created | 2019-10-21T14:34:31Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Lamers, D., Visscher, B., Weusthuis, R.A., Francke, C., Wijffels, R.H. & Lokman, C. (2019). Overexpression of delta-12 desaturase in the yeast Schwanniomyces occidentalis enhances the production of linoleic acid. Bioresource Technology, 289: 121672. doi: | en_US |
dc.identifier.issn | 1873-2976 | |
dc.identifier.uri | https://hdl.handle.net/11250/2659048 | |
dc.description.abstract | The oleaginous yeast Schwanniomyces occidentalis was previously isolated because of its excellent suitability to convert lignocellulosic hydrolysates into triacyl glycerides: it is able to use a broad range of sugars and is able to tolerate high concentrations of lignocellulosic hydrolysate inhibitors. Compared to other oleaginous yeasts S. occidentalis however produces a low content of unsaturated fatty acids. We show here that the linoleic acid content can be significantly improved by (over)expression Δ12-desaturases derived from S. occidentalis and Fusarium moniliforme. Expression was stable for the homologous expression but decreased during heterologous expression. Both homologous and heterologous expression of mCherry-Δ12-desaturase led to a 4-fold increase in linoleic acid from 0.02 g/g biomass to 0.08 g/g biomass resulting in the production of 2.23 g/L and 2.05 g/L of linoleic acid. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Overexpression of delta-12 desaturase in the yeast Schwanniomyces occidentalis enhances the production of linoleic acid | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | © 2019 The Author(s) | en_US |
dc.subject.nsi | VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472 | en_US |
dc.source.pagenumber | 8 | en_US |
dc.source.volume | 289 | en_US |
dc.source.journal | Bioresource Technology | en_US |
dc.identifier.doi | 10.1016/j.biortech.2019.121672 | |
dc.identifier.cristin | 1739132 | |
dc.description.localcode | Paid Open Access | en_US |