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dc.contributor.authorGerritzen, Matthias J.H.
dc.contributor.authorStangowez, Lilli
dc.contributor.authorvan de Waterbeemd, Bas
dc.contributor.authorMartens, Dirk E.
dc.contributor.authorWijffels, Rene Hubertus
dc.contributor.authorStork, Michiel
dc.identifier.citationGerritzen, M. J. H., Stangowez, L., van de Waterbeemd, B., Martens, D. E., Wijffels, R. H. & Stork, M. (2019). Continuous production of Neisseria meningitidis outer membrane vesicles. Applied Microbiology and Biotechnology, 103, 9401-9410. doi:en_US
dc.description.abstractOuter membrane vesicles (OMVs) are nanoparticles secreted by Gram-negative bacteria that can be used for diverse biotechnological applications. Interesting applications have been developed, where OMVs are the basis of drug delivery, enzyme carriers, adjuvants, and vaccines. Historically, OMV research has mainly focused on vaccines. Therefore, current OMV production processes have been based on batch processes. The production of OMVs in batch mode is characterized by relatively low yields and high costs. Transition of OMV production processes from batch to continuous processes could increase the volumetric productivity, reduce the production and capital costs, and result in a higher quality product. Here, we study the continuous production of Neisseria meningitidis OMVs to improve volumetric productivity. Continuous cultivation of N. meningitidis resulted in a steady state with similar high OMV concentrations as are reached in current batch processes. The steady state was reproducible and could be maintained for at least 600 h. The volumetric productivity of a continuous culture reached 4.0 × 1014 OMVs per liter culture per day, based on a dilution rate of 1/day. The tested characteristics of the OMVs did not change during the experiments showing feasibility of a continuous production process for the production of OMVs for any application.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleContinuous production of Neisseria meningitidis outer membrane vesiclesen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© 2019 The Author(s)en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470en_US
dc.source.journalApplied Microbiology and Biotechnologyen_US
dc.description.localcodePaid Open Accessen_US

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