dc.contributor.author | Patel, Deepti Manjari | |
dc.contributor.author | Kitani, Yoichiro | |
dc.contributor.author | Korsnes, Kjetil | |
dc.contributor.author | Iversen, Martin Haugmo | |
dc.contributor.author | Brinchmann, Monica Fengsrud | |
dc.date.accessioned | 2020-07-03T09:52:15Z | |
dc.date.available | 2020-07-03T09:52:15Z | |
dc.date.created | 2020-02-04T16:25:45Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Patel, D. M., Kitani, Y., Korsnes, K., Iversen, M. H. & Brinchmann, M. F. (2020). A truncated galectin-3 isolated from skin mucus of Atlantic salmon Salmo salar binds to and modulates the proteome of the gram-negative bacteria Moritella viscosa. Marine drugs, 18(2): 102. doi: | en_US |
dc.identifier.issn | 1660-3397 | |
dc.identifier.uri | https://hdl.handle.net/11250/2660673 | |
dc.description.abstract | The mucus of fish skin plays a vital role in innate immune defense. Some mucus proteins have the potential to incapacitate pathogens and/or inhibit their passage through the skin. In this study the aim was to isolate and characterize galectin(s), β-galactosides binding proteins, present in skin mucus. A novel short form of galectin-3 was isolated from Atlantic salmon skin mucus by α-lactose agarose based affinity chromatography followed by Sephadex G-15 gel filtration. Mass spectrometric analysis showed that the isolated protein was the C-terminal half of galectin-3 (galectin-3C). Galectin-3C showed calcium independent and lactose inhabitable hemagglutination, and agglutinated the Gram-negative pathogenic bacteria Moritella viscosa. Galectin-3 mRNA was highly expressed in skin and gill, followed by muscle, hindgut, spleen, stomach, foregut, head kidney, and liver. Moritella viscosa incubated with galectin-3C had a modified proteome. Proteins with changed abundance included multidrug transporter and three ribosomal proteins L7/12, S2, and S13. Overall, this study shows the isolation and characterization of a novel galectin-3 short form involved in pathogen recognition and modulation, and hence in immune defense of Atlantic salmon. | en_US |
dc.publisher | MDPI | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | A truncated galectin-3 isolated from skin mucus of Atlantic salmon Salmo salar binds to and modulates the proteome of the gram-negative bacteria Moritella viscosa | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | © 2020 The Author(s) | en_US |
dc.subject.nsi | VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 | en_US |
dc.subject.nsi | VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472 | en_US |
dc.source.pagenumber | 16 | en_US |
dc.source.volume | 18 | en_US |
dc.source.journal | Marine drugs | en_US |
dc.source.issue | 2 | en_US |
dc.identifier.doi | 10.3390/md18020102 | |
dc.identifier.cristin | 1790869 | |