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dc.contributor.authorSiriyappagouder, Prabhugouda
dc.contributor.authorGalindo-Villegas, Jorge
dc.contributor.authorDhanasiri, Anusha
dc.contributor.authorZhang, Qirui
dc.contributor.authorMulero, Victoriano
dc.contributor.authorKiron, Viswanath
dc.contributor.authorFernandes, Jorge Manuel de Oliveira
dc.identifier.citationSiriyappagouder, P., Galindo-Villegas, J., Dhanasiri, A. K. S., Zhang, Q., Mulero, V., Kiron, V. & Fernandes J. M. O. (2020). Pseudozyma priming influences expression of genes involved in metabolic pathways and immunity in zebrafish larvae. Frontiers in Immunology, 11: 978. doi:en_US
dc.description.abstractFungi, particularly yeasts, are known essential components of the host microbiota but their functional relevance in development of immunity and physiological processes of fish remains to be elucidated. In this study, we used a transcriptomic approach and a germ-free (GF) fish model to determine the response of newly hatched zebrafish larvae after 24 h exposure to Pseudozyma sp. when compared to conventionally-raised (CR) larvae. We observed 59 differentially expressed genes in Pseudozyma-exposed GF zebrafish larvae compared to their naïve control siblings. Surprisingly, in CR larvae, there was not a clear transcriptome difference between Pseudozyma-exposed and control larvae. Differentially expressed genes in GF larvae were involved in host metabolic pathways, mainly peroxisome proliferator-activated receptors, steroid hormone biosynthesis, drug metabolism and bile acid biosynthesis. We also observed a significant change in the transcript levels of immune-related genes, namely complement component 3a, galectin 2b, ubiquitin specific peptidase 21 and aquaporins. Nevertheless, we did not observe any significant response at the cellular level, since there were no differences between neutrophil migration or proliferation between control and yeast-exposed on GF larvae. Our findings reveal that exposure to Pseudozyma sp. may affect metabolic pathways and immune-related processes in germ-free zebrafish, suggesting that commensal yeast likely play a significant part in the early development of fish larvae.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titlePseudozyma priming influences expression of genes involved in metabolic pathways and immunity in zebrafish larvaeen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© 2020 The Author(s)en_US
dc.subject.nsiVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Akvakultur: 922en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474en_US
dc.source.journalFrontiers in Immunologyen_US
dc.relation.projectSpanish Ministry of Science, Innovation and Universities: BIO2014-52655-Ren_US
dc.relation.projectNordland County Council: 274/16en_US
dc.relation.projectNord university: 13011en_US
dc.relation.projectSpanish Ministry of Science, Innovation and Universities: BIO2017-84702-Ren_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal