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dc.contributor.authorOliveira, Catarina
dc.contributor.authorFatsini, Elvira
dc.contributor.authorFernandez, Ignacio
dc.contributor.authorAnjos, Catarina
dc.contributor.authorChauvigné, François
dc.contributor.authorCerdà, Joan
dc.contributor.authorMjelle, Robin
dc.contributor.authorFernandes, Jorge Manuel de Oliveira
dc.contributor.authorCabrita, Elsa
dc.date.accessioned2021-02-18T13:42:58Z
dc.date.available2021-02-18T13:42:58Z
dc.date.created2020-11-30T08:37:15Z
dc.date.issued2020
dc.identifier.citationOliveira, C. C. V., Fatsini, E., Fernandez, I., Anjos, C., Chauvigne, F., Cerda, J., Mjelle, R., Fernandes, J. M. O. & Cabrita, E. (2020). Kisspeptin Influences the Reproductive Axis and Circulating Levels of microRNAs in Senegalese Sole. International Journal of Molecular Sciences, 21(23): 9051. doi:en_US
dc.identifier.issn1422-0067
dc.identifier.urihttps://hdl.handle.net/11250/2728984
dc.description.abstractKisspeptin regulates puberty and reproduction onset, acting upstream of the brain–pituitary–gonad (HPG) axis. This study aimed to test a kisspeptin-based hormonal therapy on cultured Senegalese sole (G1) breeders, known to have reproductive dysfunctions. A single intramuscular injection of KISS2-10 decapeptide (250 µg/kg) was tested in females and males during the reproductive season, and gonad maturation, sperm motility, plasma levels of gonadotropins (Fsh and Lh) and sex steroids (11-ketotestosterone, testosterone and estradiol), as well as changes in small non-coding RNAs (sncRNAs) in plasma, were investigated. Fsh, Lh, and testosterone levels increased after kisspeptin injection in both sexes, while sperm analysis did not show differences between groups. Let7e, miR-199a-3p and miR-100-5p were differentially expressed in females, while miR-1-3p miRNA was up-regulated in kisspeptin-treated males. In silico prediction of mRNAs targeted by miRNAs revealed that kisspeptin treatment might affect paracellular transporters, regulate structural and functional polarity of cells, neural networks and intracellular trafficking in Senegalese sole females; also, DNA methylation and sphingolipid metabolism might be altered in kisspeptin-treated males. Results demonstrated that kisspeptin stimulated gonadotropin and testosterone secretion in both sexes and induced an unanticipated alteration of plasma miRNAs, opening new research venues to understand how this neuropeptide impacts in fish HPG axis.en_US
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleKisspeptin Influences the Reproductive Axis and Circulating Levels of microRNAs in Senegalese Soleen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.rights.holder© 2020 The Author(s)en_US
dc.subject.nsiVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470en_US
dc.source.pagenumber21en_US
dc.source.volume21en_US
dc.source.journalInternational Journal of Molecular Sciencesen_US
dc.source.issue23en_US
dc.identifier.doi10.3390/ijms21239051
dc.identifier.cristin1853901
dc.relation.projectERC-European Research Council: ERC/H2020/683210en_US
dc.source.articlenumber9051en_US


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