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dc.contributor.authorSuarez Ruiz, Catalina A
dc.contributor.authorBaca, Santiago Zarate
dc.contributor.authorvan den Broek, Lambertus A.M.
dc.contributor.authorvan den Berg, Corjan
dc.contributor.authorWijffels, Rene Hubertus
dc.contributor.authorEppink, Michel H.M.
dc.date.accessioned2021-02-19T14:05:41Z
dc.date.available2021-02-19T14:05:41Z
dc.date.created2020-11-02T13:10:52Z
dc.date.issued2020
dc.identifier.citationRuiz, C. A. S., Baca, S. Z., van den Broek, L. A. M., van den Berg, C., Wijffels, R. H. & Eppink, M. H. M. (2020). Selective fractionation of free glucose and starch from microalgae using aqueous two-phase systems. Algal Research, 46: 101801. doi:en_US
dc.identifier.issn2211-9264
dc.identifier.urihttps://hdl.handle.net/11250/2729299
dc.description.abstractMicroalgae are a promising source of lipids, pigments, proteins and carbohydrates, which are valuable compounds for many industries. However, optimal fractionation and valorization of all produced compounds is necessary to improve the economic viability of microalgae production. This paper aims to understand the fractionation of microalgae carbohydrates (free glucose and starch) in aqueous two-phase systems. Three aqueous two-phase systems were investigated to efficiently and mildly separate carbohydrates from disrupted Neochloris oleoabundans. This strain contains 16 w/w% of proteins, 48 w/w% total fatty acids and 27 w/w% carbohydrates when cultivated under saline water and nitrogen depletion conditions. The protein content decreases and the amount of fatty acids and carbohydrates increases notably under stress conditions and glucose becomes the main carbohydrate in this microalgae. Glucose is present in the disrupted microalgae as part of polymeric carbohydrates (starch) or in monomeric form (free glucose). With the aqueous two-phase system Polyethylene Glycol 400 - Cholinium dihydrogen phosphate (PEG400-ChDHp) microalgal free glucose is fractionated up to a recovery of 99% to the most hydrated bottom phase in a single step. Simultaneously, a recovery of 70% is reached for microalgal starch in the interface after two additional liquid-liquid extractions with PEG400-ChDHp. The final fractions obtained were free of pigments.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleSelective fractionation of free glucose and starch from microalgae using aqueous two-phase systemsen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.rights.holder© 2020 The Author(s)en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470en_US
dc.source.pagenumber9en_US
dc.source.volume46en_US
dc.source.journalAlgal Researchen_US
dc.identifier.doi10.1016/j.algal.2020.101801
dc.identifier.cristin1844120
dc.description.localcodePaid Open Accessen_US
dc.source.articlenumber101801en_US


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