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dc.contributor.authorPark, Youngjin
dc.contributor.authorZhang, Qirui
dc.contributor.authorWiegertjes, Geert Frits
dc.contributor.authorFernandes, Jorge Manuel de Oliveira
dc.contributor.authorKiron, Viswanath
dc.identifier.citationPark, Y., Zhang, Q., Wiegertjes, G. F., Fernandes, J. M. O. & Kiron, V. (2020). Adherent intestinal cells from Atlantic salmon show phagocytic ability and express macrophage-specific genes. Frontiers in Cell and Developmental Biology, 8: 580848. doi:en_US
dc.description.abstractOur knowledge of the intestinal immune system of fish is rather limited compared to mammals. Very little is known about the immune cells including the phagocytic cells in fish intestine. Hence, employing imaging flow cytometry and RNA sequencing, we studied adherent cells isolated from healthy Atlantic salmon. Phagocytic activity and selected gene expression of adherent cells from the distal intestine (adherent intestinal cells, or AIC) were compared with those from head kidney (adherent kidney cells, or AKC). Phagocytic activity of the two cell types was assessed based on the uptake of Escherichia coli BioParticlesTM. AIC showed phagocytic ability but the phagocytes were of different morphology compared to AKC. Transcriptomic analysis revealed that AIC expressed genes associated with macrophages, T cells, and endothelial cells. Heatmap analysis of selected genes indicated that the adherent cells from the two organs had apparently higher expression of macrophage-related genes. We believe that the adherent intestinal cells have phagocytic characteristics and high expression of genes commonly associated with macrophages. We envisage the possibilities for future studies on enriched populations of adherent intestinal cells.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleAdherent intestinal cells from Atlantic salmon show phagocytic ability and express macrophage-specific genesen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© 2020 The Author(s)en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Cellebiologi: 471en_US
dc.source.journalFrontiers in Cell and Developmental Biologyen_US
dc.relation.projectRegionale forskningsfond Nord-Norge: 272004en_US

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Navngivelse 4.0 Internasjonal
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