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dc.contributor.authorGao, Fengzheng
dc.contributor.authorWoolschot, Sep
dc.contributor.authorCabanelas, Iago Teles Dominguez
dc.contributor.authorWijffels, Rene Hubertus
dc.contributor.authorBarbosa, Maria J.
dc.identifier.citationGao, F., Woolschot, S., Cabanelas, I. T. D., Wijffels, R. H. & Barbosa, M. J. (2020). Light spectra as triggers for sorting improved strains of Tisochrysis lutea. Bioresource Technology, 321: 124434. doi:en_US
dc.description.abstractIt is known that microalgae respond to different light colors, but not at single-cell level. This work aimed to assess if different light colors could be used as triggers to sort over-producing cells. Six light spectra were used: red + green + blue (RGBL), blue (BL), red (RL), green (GL), blue + red (BRL) and blue + green (BGL). Fluorescence-activated cell sorting method was used to analyse single-cell fluorescence and sort cells. BGL and RGBL lead to the highest fucoxanthin production, while RL showed the lowest. Therefore, it was hypothesized that hyper-producing cells can be isolated efficiently under the adverse condition (RL). After exposure to all light colors for 14 days, the top 1% fucoxanthin producing cells were sorted. A sorted strain from RL showed higher (16–19%) growth rate and fucoxanthin productivity. This study showed how light spectra affected single-cell fucoxanthin and lipid contents and productivities. Also, it supplied an approach to sort for high-fucoxanthin or high-lipid cells.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleLight spectra as triggers for sorting improved strains of Tisochrysis luteaen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© 2020 The Author(s)en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470en_US
dc.source.journalBioresource Technologyen_US
dc.description.localcodePaid Open Accessen_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal