dc.contributor.author | Gao, Fengzheng | |
dc.contributor.author | Woolschot, Sep | |
dc.contributor.author | Cabanelas, Iago Teles Dominguez | |
dc.contributor.author | Wijffels, Rene Hubertus | |
dc.contributor.author | Barbosa, Maria J. | |
dc.date.accessioned | 2021-03-03T14:20:24Z | |
dc.date.available | 2021-03-03T14:20:24Z | |
dc.date.created | 2020-12-11T23:11:58Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Gao, F., Woolschot, S., Cabanelas, I. T. D., Wijffels, R. H. & Barbosa, M. J. (2020). Light spectra as triggers for sorting improved strains of Tisochrysis lutea. Bioresource Technology, 321: 124434. doi: | en_US |
dc.identifier.issn | 1873-2976 | |
dc.identifier.uri | https://hdl.handle.net/11250/2731488 | |
dc.description.abstract | It is known that microalgae respond to different light colors, but not at single-cell level. This work aimed to assess if different light colors could be used as triggers to sort over-producing cells. Six light spectra were used: red + green + blue (RGBL), blue (BL), red (RL), green (GL), blue + red (BRL) and blue + green (BGL). Fluorescence-activated cell sorting method was used to analyse single-cell fluorescence and sort cells. BGL and RGBL lead to the highest fucoxanthin production, while RL showed the lowest. Therefore, it was hypothesized that hyper-producing cells can be isolated efficiently under the adverse condition (RL). After exposure to all light colors for 14 days, the top 1% fucoxanthin producing cells were sorted. A sorted strain from RL showed higher (16–19%) growth rate and fucoxanthin productivity. This study showed how light spectra affected single-cell fucoxanthin and lipid contents and productivities. Also, it supplied an approach to sort for high-fucoxanthin or high-lipid cells. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Light spectra as triggers for sorting improved strains of Tisochrysis lutea | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | © 2020 The Author(s) | en_US |
dc.subject.nsi | VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 | en_US |
dc.source.pagenumber | 0 | en_US |
dc.source.volume | 321 | en_US |
dc.source.journal | Bioresource Technology | en_US |
dc.identifier.doi | 10.1016/j.biortech.2020.124434 | |
dc.identifier.cristin | 1858985 | |
dc.relation.project | EC/H2020/745754 | en_US |
dc.description.localcode | Paid Open Access | en_US |
dc.source.articlenumber | 124434 | en_US |