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dc.contributor.authorGao, Fengzheng
dc.contributor.authorTeles, Iago
dc.contributor.authorFerrer-Ledo, Narcís
dc.contributor.authorWijffels, Rene Hubertus
dc.contributor.authorBarbosa, Maria J.
dc.identifier.citationGao, F., Teles, I., Ferrer-Ledo, N., Wijffels, R. H. & Barbosa, M. J. (2020). Production and high throughput quantification of fucoxanthin and lipids in Tisochrysis lutea using single-cell fluorescence. Bioresource Technology, 318: 124104. doi:en_US
dc.description.abstractThis work aimed to investigate the accumulation of fucoxanthin and lipids in Tisochrysis lutea during growth (N+) and nitrogen-starvation (N−) and to correlate these products with single-cell emissions using fluorescence-activated cell sorting (FACS). Fucoxanthin content decreased 52.94% from N+ to N− in batch cultivation; increased 40.53% as dilution rate changed from 0.16 to 0.55 d−1 in continuous cultivation. Total lipids (N−) were constant (~250 mg/g), but the abundance of neutral lipids increased from 4.87% to 40.63%. Nile red can stain both polar and neutral lipids. However, in vivo, this differentiation is limited due to an overlapping of signals between 600 and 660 nm, caused by neutral lipids concentrations above 3.48% (W/W). Chlorophyll autofluorescence (720 nm) was reported for the first time as a proxy for fucoxanthin (R2 = 0.90) and polar lipids (R2 = 0.98). FACS can be used in high throughput quantification of pigments and lipids and to select and sort cells with high-fucoxanthin/lipids.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleProduction and high throughput quantification of fucoxanthin and lipids in Tisochrysis lutea using single-cell fluorescenceen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© 2020 The Author(s)en_US
dc.subject.nsiVDP::Teknologi: 500::Bioteknologi: 590en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Molekylærbiologi: 473en_US
dc.source.journalBioresource Technologyen_US
dc.description.localcodePaid Open Accessen_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal