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Circadian rhythmicity and photic plasticity of myosin gene transcription in fast skeletal muscle of Atlantic cod (Gadus morhua)

Lazado, Carlo Cabacang; Nagasawa, Kazue; Babiak, Igor; Kumaratunga, Palawinnage Hiruni Senari; Fernandes, Jorge M.O.
Journal article, Peer reviewed
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URI
http://hdl.handle.net/11250/279346
Date
2014
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  • Artikler, rapporter og annet (FBA) [398]
  • Publikasjoner fra Cristin - Nord universitet [1565]
Original version
Lazado, C.C., Nagasawa, K., Babiak, I., Kumaratunga, P.H.S. & Fernandes, J.M.O. (2014). Circadian rhythmicity and photic plasticity of myosin gene transcription in fast skeletal muscle of Atlantic cod (Gadus morhua). Marine Genomics, 18(A), 21-29. doi:   10.1016/j.margen.2014.04.011
Abstract
The circadian rhythm is a fundamental adaptive mechanism to the daily environmental changes experienced by many organisms, including fish. Myosins constitute a large family of contractile proteins that are essential functional components of skeletal muscle. They are known to display thermal plasticity but the influence of light on myosin expression remains to be investigated in fish. In the present study, we have examined the circadian rhythmicity and photoperiodic plasticity of myosin gene transcription in Atlantic cod (Gadus morhua) fast skeletal muscle. In silico mining of the Atlantic cod genome resulted in the identification of 76 myosins representing different classes, many of which were hitherto uncharacterized. Among the 23 skeletal muscle-expressed myosin genes, myh_tc, myh_n1, myh_n4, myo18a_2, and myo18b_2 displayed circadian rhythmicity and contained several circadian-related transcription factor binding sites (Creb, Mef2 and E-box motifs) within their putative promoter regions. Also, the circadian expression of these 5 myosins strongly correlated with the transcription pattern of clock genes in fast skeletal muscle. Under ex vivo conditions, myosin transcript levels lost their circadian rhythmicity. Nonetheless, different photoperiod regimes influenced the mRNA levels of myh_n4, myo18a_2 and myo18b_2 in fast skeletal muscle explants. Photoperiod manipulation in Atlantic cod juveniles revealed that continuous light significantly elevated mRNA levels of several myosins in fast skeletal muscle when compared to natural photoperiod. The circadian rhythmicity observed in some fast skeletal muscle myosin genes suggests that they may be under circadian clock regulation. In addition, the influence of photoperiod on their expression implies that myosins may be involved in the photic plasticity of muscle growth observed in Atlantic cod.
Description
 
Author's accepted version (post-print).
 
NOTICE: this is the author’s version of a work that was accepted for publication in Marine Genomics (2014). Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Marine Genomics (2014), 18(A). doi: http://dx.doi.org/10.1016/j.margen.2014.04.011.
 
Publisher
Elsevier

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