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dc.contributor.authorLi, Yuchuan
dc.contributor.authorKhanal, Prabhat
dc.contributor.authorNorheim, Frode
dc.contributor.authorHjorth, Marit
dc.contributor.authorBjellaas, Thomas
dc.contributor.authorDrevon, Christian A
dc.contributor.authorVaage, Ingvar Jarle
dc.contributor.authorkimmel, Alan
dc.contributor.authorDalen, Knut Tomas
dc.date.accessioned2022-01-25T09:55:47Z
dc.date.available2022-01-25T09:55:47Z
dc.date.created2021-04-22T16:23:45Z
dc.date.issued2021
dc.identifier.citationLi, Y., Khanal, P., Norheim, F., Hjorth, M., Bjellaas, T., Drevon, C. A., Vaage, I. J., Kimmel, A. & Dalen, K. T. (2021). Plin2 deletion increases cholesteryl ester lipid droplet content and disturbs cholesterol balance in adrenal cortex. Journal of Lipid Research, 62: 100048. doi:en_US
dc.identifier.issn1539-7262
dc.identifier.urihttps://hdl.handle.net/11250/2839147
dc.description.abstractCholesteryl esters (CEs) are the water-insoluble transport and storage form of cholesterol. Steroidogenic cells primarily store CEs in cytoplasmic lipid droplet (LD) organelles, as contrasted to the majority of mammalian cell types that predominantly store triacylglycerol (TAG) in LDs. The LD-binding Plin2 binds to both CE- and TAG-rich LDs, and although Plin2 is known to regulate degradation of TAG-rich LDs, its role for regulation of CE-rich LDs is unclear. To investigate the role of Plin2 in the regulation of CE-rich LDs, we performed histological and molecular characterization of adrenal glands from Plin2+/+ and Plin2−/− mice. Adrenal glands of Plin2−/− mice had significantly enlarged organ size, increased size and numbers of CE-rich LDs in cortical cells, elevated cellular unesterified cholesterol levels, and increased expression of macrophage markers and genes facilitating reverse cholesterol transport. Despite altered LD storage, mobilization of adrenal LDs and secretion of corticosterone induced by adrenocorticotropic hormone stimulation or starvation were similar in Plin2+/+ and Plin2−/− mice. Plin2−/− adrenals accumulated ceroid-like structures rich in multilamellar bodies in the adrenal cortex-medulla boundary, which increased with age, particularly in females. Finally, Plin2−/− mice displayed unexpectedly high levels of phosphatidylglycerols, which directly paralleled the accumulation of these ceroid-like structures. Our findings demonstrate an important role of Plin2 for regulation of CE-rich LDs and cellular cholesterol balance in the adrenal cortex.en_US
dc.language.isoengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titlePlin2 deletion increases cholesteryl ester lipid droplet content and disturbs cholesterol balance in adrenal cortexen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.rights.holder© 2021 The Author(s)en_US
dc.subject.nsiVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.source.volume62en_US
dc.source.journalJournal of Lipid Researchen_US
dc.identifier.doi10.1016/j.jlr.2021.100048
dc.identifier.cristin1905906
dc.source.articlenumber100048en_US


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