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dc.contributor.authorMonzón‑Atienza, Luis
dc.contributor.authorBravo, Jimena
dc.contributor.authorFernández-Montero, Álvaro
dc.contributor.authorCharlie-Silva, Ives
dc.contributor.authorMontero, Daniel
dc.contributor.authorRamos-Vivas, José
dc.contributor.authorGalindo-Villegas, Jorge
dc.contributor.authorAcosta, Félix
dc.date.accessioned2022-06-22T11:33:37Z
dc.date.available2022-06-22T11:33:37Z
dc.date.created2022-03-22T14:04:02Z
dc.date.issued2022
dc.identifier.citationMonzón‑Atienza, L., Bravo, J., Fernández-Montero, Á., Charlie-Silva, I., Montero, D., Ramos-Vivas, J., Galindo-Villegas, J. & Acosta, F. (2022). Dietary supplementation of Bacillus velezensis improves Vibrio anguillarum clearance in European sea bass by activating essential innate immune mechanisms. Fish and Shellfish Immunology, 124, 244-253. doi:en_US
dc.identifier.issn1095-9947
dc.identifier.urihttps://hdl.handle.net/11250/3000048
dc.description.abstractBacillus spp. supplementation as probiotics in cultured fish diets has a long history of safe and effective use. Specifically, B. velezensis show great promise in fine-tuning the European sea bass disease resistance against the pathogenicity caused by several members of the Vibrio family. However, the immunomodulatory mechanisms behind this response remain poorly understood. Here, to examine the inherent immune variations in sea bass, two equal groups were fed for 30 days with a steady diet, with one treatment supplemented with B. velezensis. The serum bactericidal capacity against live cells of Vibrio anguillarum strain 507 and the nitric oxide and lysozyme lytic activities were assayed. At the cellular level, the phagocytic response of peripheral blood leukocytes against inactivated Candida albicans was determined. Moreover, head-kidney (HK) total leukocytes were isolated from previously in vivo treated fish with LPS of V. anguillarum strain 507. Mechanistically, the expression of some essential proinflammatory genes interleukin-1 (il1b), tumor necrosis factor-alpha (tnfa), and cyclooxygenase 2 (cox2) and the sea bass specific antimicrobial peptide (AMP) dicentracin (dic) expressions were assessed. Surprisingly, the probiotic supplementation significantly increased all humoral lytic and cellular activities assayed in the treated sea bass. In addition, time-dependent differences were observed between the control and probiotic treated groups for all the HK genes markers subjected to the sublethal LPS dose. Although the il1b was the fastest responding gene to a significant level at 48 hours post-injection (hpi), all the other genes followed 72h in the probiotic supplemented group. Finally, an in vivo bacteria challenge against live V. anguillarum was conducted. The probiotic fed fish observed a significantly higher survival. Overall, our results provide clear vertical evidence on the beneficial immune effects of B. velezensis and unveil some fundamental immune mechanismen_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleDietary supplementation of Bacillus velezensis improves Vibrio anguillarum clearance in European sea bass by activating essential innate immune mechanismsen_US
dc.title.alternativeDietary supplementation of Bacillus velezensis improves Vibrio anguillarum clearance in European sea bass by activating essential innate immune mechanismsen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.rights.holder© 2022 The Author(s)en_US
dc.subject.nsiVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Akvakultur: 922en_US
dc.subject.nsiVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923en_US
dc.source.pagenumber244-253en_US
dc.source.volume124en_US
dc.source.journalFish and Shellfish Immunologyen_US
dc.identifier.doi10.1016/j.fsi.2022.03.032
dc.identifier.cristin2011737


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