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dc.contributor.authorHesp, Kylie
dc.contributor.authorAlvarez, John L. Flores
dc.contributor.authorAlexandru, Ana-Maria
dc.contributor.authorvan der Linden, Jip
dc.contributor.authorMartens, Dirk E.
dc.contributor.authorWijffels, Rene Hubertus
dc.contributor.authorPomponi, Shirley A.
dc.identifier.citationHesp, K., Alvarez, J. L. F., Alexandru, A.-M., van der Linden, J., Martens, D. E., Wijffels, R. H. & Pomponi, S. A. (2020). CRISPR/Cas12a-mediated gene editing in Geodia barretti sponge cell culture. Frontiers in Marine Science, 7: 599825. doi:en_US
dc.description.abstractSponges and their associated microorganisms are the most prolific source of marine natural products, and many attempts have been made at creating a marine sponge cell line to produce these products efficiently. However, limited knowledge on the nutrients sponge cells require to grow and poor genetic accessibility have hampered progress toward this goal. Recently, a new sponge-specific nutrient medium M1 has been shown to stimulate sponge cells in vitro to divide rapidly. In this study, we demonstrate for the first time that sponge cells growing in M1 can be genetically modified using a CRISPR/Cas12a gene editing system. A short sequence of scrambled DNA was inserted using a single-stranded oligodeoxynucleotide donor template to disrupt the 2′,5′-oligoadenylate synthetase gene of cells from the boreal deep-sea sponge Geodia barretti. A blue fluorescent marker gene appeared to be inserted in an intron of the same gene and expressed by a small number of G. barretti cells. Our results represent an important step toward developing an optimized continuous sponge cell line to produce bioactive compounds.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleCRISPR/Cas12a-mediated gene editing in Geodia barretti sponge cell cultureen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© 2020 The Author(s)en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474en_US
dc.subject.nsiVDP::Teknologi: 500::Bioteknologi: 590en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497en_US
dc.source.journalFrontiers in Marine Scienceen_US

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