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dc.contributor.authorBeirão, José
dc.contributor.authorFlengstad, Stian
dc.contributor.authorBabiak, Igor
dc.identifier.citationBeirão, J., Flengstad, S. & Babiak, I. (2020). Spotted wolffish (Anarhichas minor) sperm cryopreservation in 5-mL cryovials. Fish Physiology & Biochemistry. doi:en_US
dc.description.abstractIn spotted wolffish Anarhichas minor aquaculture, cryopreservation is used to secure sperm availability throughout the entire spawning season. Under current protocols, sperm is cryopreserved in 0.5-mL straws. This implies thawing a considerable number of straws for insemination with cryopreserved sperm. In this work, we scale up the spotted wolffish sperm cryopreservation procedure through the development of a protocol for sperm cryopreservation in 5-mL cryovials. Different freezing (distances from the liquid nitrogen surface) and thawing rates were tested. The best results were obtained with cryovials frozen at a distance of 1.5 cm from the liquid nitrogen surface and thawed either at 15 or 10 °C for 4 and 6 min, respectively. Under these conditions, similar percentage of motile cells, sperm velocity and percentage of viable cells were obtained in comparison with the sperm cryopreserved in the traditional 0.5-mL straws. This protocol will facilitate the process of insemination with cryopreserved sperm in the spotted wolffish hatcheries.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleSpotted wolffish (Anarhichas minor) sperm cryopreservation in 5-mL cryovialsen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© 2020 The Author(s)en_US
dc.subject.nsiVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Akvakultur: 922en_US
dc.subject.nsiVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Embryologi: 482en_US
dc.source.journalFish Physiology & Biochemistryen_US
dc.relation.projectMABIT: AF0078en_US
dc.description.localcodeUnit License Agreementen_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal