Epigenetic mapping of the somatotropic axis in Nile tilapia reveals differential DNA hydroxymethylation marks associated with growth
Konstantinidis, Ioannis; Anastasiadi, Dafni; Sætrom, Pål; Nedoluzhko, Artem; Mjelle, Robin; Podgorniak, Tomasz; Piferrer, Francesc; Fernandes, Jorge Manuel de Oliveira
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2021Metadata
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Konstantinidis, I., Anastasiadi, D., Sætrom, P., Nedoluzhko, A. V., Mjelle, R., Podgorniak, T., Piferrer, F. & Fernandes, J. M. O. (2021). Epigenetic mapping of the somatotropic axis in Nile tilapia reveals differential DNA hydroxymethylation marks associated with growth. Genomics, 113(5), 2953-2964. doi: 10.1016/j.ygeno.2021.06.037Abstract
In vertebrates, the somatotropic axis comprising the pituitary gland, liver and muscle plays a major role in myogenesis. Its output in terms of muscle growth is highly affected by nutritional and environmental cues, and thus likely epigenetically regulated. Hydroxymethylation is emerging as a DNA modification that modulates gene expression but a holistic characterization of the hydroxymethylome of the somatotropic axis has not been investigated to date. Using reduced representation 5-hydroxymethylcytosine profiling we demonstrate tissue-specific localization of 5-hydroxymethylcytosines at single nucleotide resolution. Their abundance within gene bodies and promoters of several growth-related genes supports their pertinent role in gene regulation. We propose that cytosine hydroxymethylation may contribute to the phenotypic plasticity of growth through epigenetic regulation of the somatotropic axis.