Photobiomodulation reduces the cytokine storm syndrome associated with Covid-19 in the zebrafish model
Rosa, Ivana F.; Peçanha, Ana Paula B.; Carvalho, Tábata R. B.; Alexandre, Leonardo S.; Ferreira, Vinícius G.; Doretto, Lucas B.; Souza, Beatriz M.; Nakajima, Rafael T.; da Silva, Patrick; Barbosa, Ana P.; Gomes-de-Pontes, Leticia; Bomfim, Camila G.; Machado-Santelli, Glaucia M.; Condino-Neto, Antonio; Guzzo, Cristiane R.; Peron, Jean P. S.; Andrade-Silva, Magaiver; Câmara, Niels O. S.; Garnique, Anali M. B.; Medeiros, Renata J.; Ferraris, Fausto K.; Barcellos, Leonardo J. G.; Correia-Junior, Jose D.; Galindo-Villegas, Jorge; Machado, Mônica F. R.; Castoldi, Angela; Oliveira, Susana L.; Costa, Camila C.; Belo, Marco A. A.; Galdino, Giovane; Sgro, Germán G.; Bueno, Natalia F.; Eto, Silas F.; Veras, Flávio P.; Fernandes, Bianca H. V.; Sanches, Paulo R. S.; Cilli, Eduardo M.; Malafaia, Guilherme; Nóbrega, Rafael H.; Garcez, Aguinaldo S.; Carrilho, Emanuel; Charlie-Silva, Ives
Peer reviewed, Journal article
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Original versionRosa, I. F., Peçanha, A. P. B., Carvalho, T. R. B., Alexandre, L. S., Ferreira, V. G., Doretto, L. B., Souza, B. M., Nakajima, R. T., da Silva, P., Barbosa, A. P., Gomes-de-Pontes, L., Bomfim, C. G., Machado-Santelli, G. M., Condino-Neto, A., Guzzo, C. R., Peron, J. P. S., Andrade-Silva, M., Câmara, Niels O. S., Garnique, A. M. B., ... Charlie-Silva, I. (2023). Photobiomodulation reduces the cytokine storm syndrome associated with Covid-19 in the zebrafish model. International Journal of Molecular Sciences, 24(7), Article 6104. doi: 10.3390/ijms24076104
Although the exact mechanism of the pathogenesis of COVID-19 is not fully understood, oxidative stress and the release of pro-inflammatory cytokines have been highlighted as playing a vital role in the pathogenesis of the disease. In this sense, alternative treatments are needed to reduce the inflammation caused by COVID-19. Therefore, this study aimed to investigate the potential effect of red PBM as an attractive therapy to downregulate the cytokine storm caused by COVID-19 from a zebrafish model. RT-PCR analyses and protein-protein interaction prediction among SARS-CoV-2 and Danio rerio proteins showed that rSpike was responsible for generating systemic inflammatory processes with significantly increased pro-inflammatory (il1b, il6, tnfa, and nfkbiab), oxidative stress (romo1) and energy metabolism (slc2a1a, coa1) mRNA markers, with a pattern like those observed in COVID-19 cases in humans. On the other hand, PBM treatment decreased the mRNA levels of these pro-inflammatory and oxidative stress markers compared with rSpike in various tissues, promoting an anti-inflammatory response. Conversely, PBM promotes cellular and tissue repair of injured tissues and significantly increases the survival rate of rSpike-inoculated individuals. Additionally, metabolomics analysis showed that the most impacted metabolic pathways between PBM and the rSpike-treated groups were related to steroid metabolism, immune system, and lipids metabolism. Together, our findings suggest that the inflammatory process is an incisive feature of COVID-19, and red PBM can be used as a novel therapeutic agent for COVID-19 by regulating the inflammatory response. Nevertheless, the need for more clinical trials remains, and there is a significant gap to overcome before clinical trials.